com.apple.windowserver.plist file

Hello, I was just wondering is it safe to trash com.apple.windowserver.plist

file located on library/preferences/

after reboot is it going to be initiated?

Why would you want to do that? Messing with system plists is iffy....

if you must, make a copy of it somewhere else, and delete it, if it resetts then youre ok, if not replace it with the backup